Figure 4. Changes in H3K36me2 status in porcine oocytes and parthenogenetic embryos.

(A) Oocytes and parthenogenetic embryos were immunostained with the anti-H3K36me2 antibody, which was then localized with a FITC-conjugated secondary antibody (green). DNA was stained with DAPI (blue). NSN and SN are two types of GV stage oocytes. MI, metaphase I stage oocytes; MII, metaphase II stage oocytes. Scale bar = 20 µm. (B) Relative intensities of fluorescence signals from H3K36me2. Total 15 oocytes and 22 embryos were analyzed in triplicate in this experiment. Bars represent least-squares showed the standard error in each group. P-values <0.05 were considered statistically significant. There was no significant difference among all groups.