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. 2014 Jun 7;14:149. doi: 10.1186/1471-2180-14-149

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Copyright © 2014 Mei et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Physical map of the sequences flanking the emetic gene clusters. About 5 kb DNA sequences upstream of cesH and downstream of cesD were analyzed for CER057, CER074, BtB2-4, IS075 and F4810/75, respectively, and due to the available sequences are shorter, about 5 kb DNA sequences upstream of cesH and 2.2 kb downstream of cesD were analyzed for MC67 and MC118. The composite transposon Tnces in emetic B. weihenstephanensis MC67 and MC118 is indicated by black triangles. The Tnces consists of ces gene cluster flanked by two copies of IS element at each end in the opposite direction, containing a transposase gene and 16 bp invert repeats (IRL and IRR) at both ends. Sign and color codes are indicated on the right hand side. Physical map is not at scale.