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. 2014 Jun 7;14:149. doi: 10.1186/1471-2180-14-149

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Copyright © 2014 Mei et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Southern blot hybridization analysis of the transconjugants of Tn ces ::km transposition in E. coli HB101. Two independent hybridizations were performed. A: lane 1–4, independent Km^RSm^R transconjugants, lane 5, HB101, lane 6, JM109 (pTnkm); and B: lane 1–5, independent Km^RSm^R transconjugants, lane 6, HB101, lane 7, JM109 (pTnkm). Three probes of Km (a), ISces (b) and blapuc18 (c), respectively were used for hybridization as illustrated in Figure 4.