Table 3.
Primers used in this study
| Primers | Target | Sequences (5’ → 3’) |
|---|---|---|
| EmF |
cesB |
GACAAGAGAAATTTCTACGAGCAAGTACAAT |
| EmR |
|
GCAGCCTTCCAATTACTCCTTCTGCCACAGT |
| 14 F |
pXO1-14 |
GGTAAAGAGTGCGGAAAATGA |
| 14R |
|
AATACGCCAACGCCAACTTA |
| 45 F |
pXO1-45 |
TGCAGCTCGTAATCCACAG |
| 45R |
|
TGCTAATGATAAAACGCCTGG |
| 50 F |
pXO1-50 |
TTCGTACAGATGAAACACAGG |
| 50R |
|
GTGCCTCAAGATGAACCTTC |
| 55 F |
pXO1-55 |
GATAGAGACTGCTCTTGGGAA |
| 55R |
|
GGTCTTAGCCATGAGAGTAAAAACA |
| 58 F |
pXO1-58 |
TGTGATGGACCTTTGTATTAATTTGT |
| 58R |
|
ATACCCCGCATGGAGCTTAG |
| ISF_SacI |
ISces |
GCAGAGCTCGGTTCTGGTGCAAAAACTTCAGGACA |
| ISR_XbaI |
|
GCATCTAGAGGTTCTGGTGCAAAAAGATAATAAAG |
| ISF_HindIII |
ISces |
GCAAAGCTTGGTTCTGGTGCAAAAACTTCAGGACA |
| ISR_BamHI |
|
GCAGGATCCGGTTCTGGTGCAAAAAGATAATAAAG |
| KmF_XbaI |
Km |
TCATCTAGATAAACCCAGCGAACCATTTG |
| KmR_BamHI |
|
TCAGGATCCTCTAGGTACTAAAACAATTCATCCAG |
| ISF3 |
ISces |
TCTGGTGCAAAAACTTCAGG |
| ISR3 |
|
AAGTCGCATACGACCAGGTA |
| kmF3 |
Km |
GAGGATGAGGAGGCAGATTG |
| KmR3 |
|
CGGCCAGATCGTTATTCAGT |
| APF1 |
bla |
TTTGCCTTCCTGTTTTTGCT |
| APR1 |
|
TTGCCGGGAAGCTAGAGTAA |
| ISL-SP1 |
CTTCATCCTCTTCGTCTTGGTAGC |
|
| ISL-SP2 |
GGTTCGCTGGGTTTATCTAGAGGT |
|
| ISL-SP3 |
GACAGACTGGTCCCGTAAATCAAC |
|
| ISR-SP1 |
ATATCGGGGAAGAACAGTATGTCG |
|
| ISR-SP2 |
GTACCTAGAGGATCCGGTTCTGGT |
|
| ISR-SP3 |
GACAGACTGGTCCCGTAAATCAAC |
|
| IS-LR |
CTTTCGAATCAACAGCACGA |
|
| CesD-SP1 |
GGCCTATTGTATAATGACAACG |
|
| CesD-SP2 |
GGTGTATTATTTATCTTCGCCTG |
|
| CesD-SP3 |
GGTATTTTAGGGGCGAAGGTTC |
|
| MH-SP1 |
CACTCTTGCGTTTTTGCGTATC |
|
| MH-SP2 |
AAACAATGAGCCCACCCCGAAA |
|
| MH-SP3 | CGCTTTTCCACATTCTTTACGG | |