Table II.
CD8+ T cell AV9+ TCR CDR3 diversity
| Mouse Strains | AV9 Segment Usage | CDR3 Diversity |
|---|---|---|
| C57BL/6 | 42/45a | |
| 9D-4b | 28 | |
| 9-4 | 14 | |
| A*01:03 | 28/28 | |
| 9D-4 | 14 | |
| 9N-3 | 12 | |
| 9N-4 | 1 | |
| 9D-1 | 1 | |
| A*24:02 | 19/20 | |
| 9N-2 | 12 | |
| 9D-1 | 5 | |
| 9N-3 | 2 | |
| B*08:01 | 27/32 | |
| 9N-3 | 17 | |
| 9D-4 | 8 | |
| 9N-4 | 3 | |
| 9D-1 | 3 | |
| 9N-2 | 1 | |
| B*27:05 | 36/37 | |
| 9N-3 | 21 | |
| 9D-4 | 7 | |
| 9N-4 | 6 | |
| 9D-1 | 2 | |
| 9D-2 | 1 | |
| B*35:01 | 20/20 | |
| 9N-3 | 12 | |
| 9N-2 | 7 | |
| 9-4 | 1 | |
| B*44:02 | 19/19 | |
| 9D-4 | 11 | |
| 9N-3 | 4 | |
| 9-4 | 2 | |
| 9N-2 | 1 | |
| 9D-1 | 1 | |
| C*07:01 | 25/32 | |
| 9N-3 | 18 | |
| 9D-4 | 10 | |
| 9D-1 | 4 | |
| 9-4 | 2 |
After purification of CD8+ T cells, the CDR3 subregions of AV9+ TCR α-chains from each of the HLA monochain transgenic strains were RT-PCR amplified, cloned, and sequenced; AV9+ TCR α-chains of C57BL/6 WT mice were similarly analyzed.
a
Number of different CDR3 sequences versus the total number of in-frame sequences analyzed.
b
Usage of the individual variable segments of the AV9 family.