Table I.
Potential HLA-A*0201–restricted epitopes selected from HSV gB
| Peptide | Sequence | Molecular Mass (kDa) | Amino Acids (No.) | BIMAS | SYFPEITHI | MAPPP | MHCPred |
|---|---|---|---|---|---|---|---|
| gB17–25 | ALLGLTLGV | 856.0 | 9 | 257.342 | 31 | 0.5241 | 0.27 |
| gB161–169 | TMYYKDVTV | 1119.3 | 9 | 160.742 | 23 | 0.8421 | 0.34 |
| gB183–191 | GIFEDRAPV | 1003.1 | 9 | 145.077 | 24 | 0.5003 | 0.24 |
| gB286–294 | FVLATGDFV | 968.1 | 9 | 279.149 | 17 | — | 0.09 |
| gB342–350 | NLLTTPKFT | 1034.2 | 9 | 151.648 | 15 | — | — |
| gB343–351 | LLTTPKFTV | 1019.2 | 9 | 685.783 | 24 | 1 | 0.19 |
| gB441–449 | YLANGGFLI | 967.1 | 9 | 278.347 | 24 | — | 0.2 |
| gB447–455 | FLIAYQPLL | 1077.3 | 9 | 98.267 | 25 | 0.8300 | 0.4 |
| gB561–569 | RMLGDVMAV | 991.2 | 9 | 427.474 | 27 | 0.7222 | 0.37 |
| gB675–683 | TMLEDHEFV | 1120.2 | 9 | 2053.642 | 22 | 0.9942 | 0.19 |
The sequence of HSV-1 glycoprotein B was submitted to screening of potential HLA-A*0201 epitopes using several computer algorithms. Ten peptides were selected on the basis of HLA-A*0201 binding motif sequence from HSV-1 gB. The numbers in the four right columns show predicted IC50 as calculated by BIMAS (http://www-bimas.cit.nih.gov/molbio/hla_bind), SYFPEITHI http://www.syfpeithi.de), MAPPP (http://www.mpiib-berlin.mpg.de/MAPPP), and MHCPred (http://www.mhc-pathway.net). The sequences of synthesized peptides are based on the HSV-1 strain 17.