Figure 1.

γδ T lymphocytes migrate toward LTB₄. (A) In vivo γδ T lymphocyte accumulation in C57BL/6 mouse pleural cavities triggered by i.pl. injection of LTB₄ (500 ng/cavity) 24 h after stimulation. Results are expressed as the mean ± sem from at least five animals/group in three different experiments. (B) In vitro chemotaxis of γδ T lymphocytes induced by LTB₄ (100 nM). Spleen leukocytes (3×10⁶/well) were placed in the upper chamber of 3 μm pore diameter transwell inserts and allowed to transmigrate toward LTB₄ for 2 h. Cells that migrated into the bottom chamber were recovered, counted, and labeled for γδ TCR for FACS analysis as described in Materials and Methods. Results are expressed as the chemotactic index, and accumulation in response to vehicle had a chemotactic index of 1, as mean ± sem from triplicate values of a representative experiment out of three separate experiments. *, Statistically significant differences (P≤0.05).