Figure 4.

LPS-triggered γδ T lymphocyte accumulation requires BLT1. (A) In vivo pretreatment of mice with CP105,696 (4 mg/kg, i.p.) or with LY292476 (20 mg/kg, s.c.) impaired γδ T cell accumulation in C57BL/6 mice pleural cavities 24 h after LPS i.pl. (250 ng/cavity) stimulation. Results are expressed as the mean ± sem from at least eight animals/group in three separate experiments. (B) BLT1 blockade by CP105,696 (1 μM) inhibited in vitro γδ T lymphocyte migration induced by LPW. Spleen leukocytes (3×10⁶/well) were placed in the upper chamber of 3 μm pore diameter transwell inserts and allowed to transmigrate toward LPW for 2 h. Migrated cells recovered from the bottom chamber were counted and labeled for FACS analysis. SPW were used as control. Results are expressed as the chemotactic index, and accumulation in response to vehicle had a chemotactic index of 1 as mean ± sem from triplicate wells from one out of three separate experiments. *, Statistically significant differences (P≤0.05) between LPW- and SPW-stimulated and stimulated groups; +, significant differences between stimulated and treated groups.