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Fluorescence images of HCT 116 cells after incubation with medium containing PEI/DNA binary and ASF/PEI/DNA ternary complexes in the absence of FBS at N/P ratios of 8/1 (A); 12/1 (B) and 15/1 (C). After a 6 h incubation, the medium was replaced with fresh DMEM containing 10% FBS, followed by incubation for 24 h. Then the micrographs were obtained with a magnification of 100×.
