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. 2014 Apr 29;15(5):7398–7408. doi: 10.3390/ijms15057398

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — Western blot of EN2 overexpression lysates. (A) EN2 protein levels performed by ab45867 (left) and ab28731 (right) (lane 1: Lysates of HK2 cells, lane 2: Lysates of untransfected HEK 293T cells, lane 3: Lysates of EN2 overexpression in HEK 293T cells); (B) Indirect ELISA for peptide detection with the amounts of synthesized peptides increased with 2-fold change, * represents p < 0.05 when compared with the blank control. (C) qPCR analysis of lentiviral-mediated EN2 RNAi knockdown in 786-O cells (lane 1: Negative Control, lane 2: 1# siRNA, lane 3: 2# siRNA, lane 4: 3# siRNA); (D) Western blot analysis of lentiviral-mediated EN2 RNAi knockdown in 786-O cells (lane 1: Negative Control, lane 2: 1# siRNA, lane 3: 2# siRNA, lane 4: 3# siRNA); and (E) Semi-quantitative western blot analysis of EN2 RNAi knockdown. * represents p < 0.05 when compared with the lane 1.