Table 3.
The conserved Ca2+-binding site in CAPN large subunit (DI-DIV) with protein structures.
| PDB ID | Molecular description | The Ca2+ binding site | The residue conservation pattern * |
|---|---|---|---|
| 1KXR | Ca2+ bound protease core of CAPN1(rat) | Site 1: Val99, Gly101, Asp106, Glu185 Site 2: Glu302, Asp309, Met 329, Asp331, Glu333 |
Site 1: VGDE Site 2: EDMDE Site 3: ADEE |
| 1ZCM | CAPN1 protease core inhibited by ZLLYCH2F(human) | Site 1: Val99, Gly101, Asp106, Glu185 Site 2: Glu302, ASP309, Met329, Asp331, Glu333 |
|
| Activated CAPN1 structure model (bovine) | Site 1: Val99, Asp100, Gly101, Thr103, Asp106, Glu185 Site 2: Glu302, ASP309, Met329, Asp331, Glu333 Site 3: Ala557, Asp560, Glu562, Glu567 |
||
| 3BOW | Complex of CAPN2 and CAST(rat) | Site 1: Ile89, Gly90, Gly91, Asp96, Glu175 Site2: Glu292, Asp299, Gln319, Asp321, Glu323 Site3: Ala542, Asp545, Glu547, Glu552 Site 4: Asp585, Asp587, Ser589, Lys591, Glu596 Site 5: Asp615, Asp617, Ser618, Thr621, Glu626 Site 6: Asp658, Asn661 |
Site 1: IGGDE Site 2: EDQDE Site 3: ADEE Site 4: DDSLE Site 5:DDRTE Site 6:DDDN |
| 3DF0 | Complex of CAPN2 and CAST(rat) | Site 1: Ile89, Gly90, Gly91, Asp96, Glu175 Site2: Glu292, Asp299, Gln319, Asp321, Glu323 Site 3: Ala542, Asp545, Ala546, Glu547, Glu552 Site 4: Glu547, Asp585, Asp587, Ser589, Lys591, Glu596 Site 5: Asp615, Asp617, Ser618, Thr621, Glu626 Site 6: Asp570, Asp658, Asp660, Asn661 |
*
The presence of Ca2+ ions bound in the conservation patterns of CAPN large subunit with protein structure alignment in which residues are conserved between CAPN1 and CAPN2 represented by black, class-specific residues are indicated in blue and pink for CAPN1 and CAPN2, respectively.