Table 2.

qPCR primer sequences of selected genes.

Gene	Sequence 5′ to 3′	Accession	Position (bp)	Exon-exon overlap	% Primer efficiency
Srebf1-F	CTGGCTTGGTGATGCTATGTTG	NM_011480.3	3901–3922	No	104.9
Srebf1-R	GACCATCAAGGCCCCTCAA		3978–3960	No
Hspa5-F	GTGGAGATCATAGCCAACG	NM 022310.3	383–401	No	102.9
Hspa5-R	CACATACGACGGCGTGATGC		433–414	No
Cpt1a-F	GGAGGAGACAGACACCATCCA	NM 013495.2	1044–1064	Yes	104.1
Cpt1a-R	CGTCATGGTAGAGCCAGACCTT		1138–1117	No
Pparα-F	CGATGCTGTCCTCCTTGATGA	NM_011144.6	1401–1421	No	98.5
Pparα-R	GAAGTCAAACTTGGGTTCCATGAT		1528–1505	No
Dnmt1-F	CCAGCTGCCAAACGGAGA	NM_001199431.1	1060–1077	Yes	102
Dnmt1-R	CCTCGGGAGTCTCTGGAGCTA		1123–1103	No
Dnmt3a-F	CACTGGAGTAGGCGCTGAGAC	NM_007872.4	7635–7655	No	101.6
Dnmt3a-R	CAGCAAAGGGCCTTCCATAG		7699–7680	No
Dnmt3b-F	CCGTTCGACTTGGTGATTGG	NM_001003961.4	2352–2371	No	101.3
Dnmt3b-R	GGGCAGGATTGACGTTAGAGAG		2412–2391	No
Ahcy-F	ATCCTTGGCCGGCACTTT	NM_016661.3	949–966	Yes	97.1
Ahcy-R	TTCTTTAGCCAGTAGCGGTCCA		1100–1079	No
Mat1a-F	TCTGTCCCATACTCACCTCTTCAG	NM_133653	1683–1706	No	98.4
Mat1a-R	TGCCCTGAGGGTAGAAGGC		1771–1753	No
Mat2a-F	CAGGAGACCAGGGTTTGATGTT	NM_145569	517–538	Yes	95.8
Mat2a-R	GCGTAACCAAGGCAATGTACC		653–633	No
Mtr-F	CTGCAGATGTGGCCAGAAAAG	NM_001081128	580–600	Yes	98.9
Mtr-R	CAGCCACAAACCTCTTGACTCC		648–627	No