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. 2014 May 9;15(5):8256–8279. doi: 10.3390/ijms15058256

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 4. — CID spectrum of the PC standard (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) of protonated (top) and sodiated (below) molecules, recorded under NP LC/MS conditions (phase A—hexane/isopropanol (3:2, v/v) and phase B—an isopropanol/hexane/5 mM aqua solution of ammonium acetate (38:56:5, v/v/v). The following elution program was employed: from 53% B to 80% B for 23 min, 80% B maintained for 4 min, 80% B to 100% B for 9 min and 100% B maintained for 14 min. The flow rate was 1 mL/min) in the positive ion MS mode.