Figure 8.
Effects of live and temperature-treated L. dumoffii cells cultured on the choline-supplemented medium and without choline on the ability to induce TNF-α production in THP-1 differentiated cells. Data represent the mean ± S.D. of five independent experiments. Control THP-1 differentiated cells (PMA-activated): TNF-α 22.6 ± 0.6 (pg/mL); Control non-activated THP-1 cells: TNF-α 18.32 ± 0.9 (pg/mL). # Significantly different from the lower bacteria dose, p ≤ 0.05; * Statistically significant in comparison to bacteria cultured on the choline non-supplemented medium, p ≤ 0.05 (ANOVA and Tukey’s post hoc test). a,b Statistically significant in comparison with similar experiments preformed with temperature-treated bacteria (a
p ≤ 0.05, b
p ≤ 0.01) c (ANOVA and Tukey’s post hoc test)
—live L. dumoffii;
—temperature-treated L. dumoffii;
—live L. dumoffii cultured on choline-supplemented medium;
—temperature-treated L. dumoffii cultured on choline-supplemented medium.