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. 2014 May 16;15(5):8773–8794. doi: 10.3390/ijms15058773

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 2. — Wound healing assay of H1_DL2 melanoma brain metastasis cells after treatment with vemurafenib and temsirolimus. (A) Untreated H1_DL2 cells at 0 h; (B) Untreated cells 60 h after initiating a scratch wound; (C) Representative picture 60 h after initiating a scratch wound and starting the treatment with 5 μM of temsirolimus; (D) Representative picture 60 h after initiating a scratch wound and starting the treatment with 5 μM of vemurafenib; (E) Representative picture 48 h after initiating a scratch wound and starting combined treatment (5 μM temsirolimus and 5 μM vemurafenib); and (F) Representative picture 60 h after initiating a scratch wound and starting combined treatment (5 μM temsirolimus and 5 μM vemurafenib). Scale bar = 200 μm.