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. 2014 May 19;15(5):8863–8877. doi: 10.3390/ijms15058863

Figure 5.

Figure 5.

Effects of sulfuretin on Nrf2 nuclear translocation (A), antioxidant response element (ARE) activation (B), and transfection with Nrf2 siRNA (C) in human liver-derived HepG2 cells. (A) Cells were treated with 40 μM sulfuretin for 0–120 min. The nuclei were fractionated from the cytosol using PER Mammalian Protein Extraction Buffer as described in the Experimental section; (B) Quiescent cells transiently transfected with ARE-luciferase or control vector were incubated for 1 h with the indicated concentrations of sulfuretin in the presence of 5% fetal bovine serum (FBS). Cell lysates were assayed for the fold induction of luciferase activity by normalizing the transfection efficiency and dividing the values of each experiment relative to the control; and (C) Cells were transiently transfected with Nrf2 siRNA, and then treated with 40 μM of sulfuretin for 12 h. Nrf2 and HO-1 protein were detected by western blot analysis, and representative blots from three independent experiments with similar results. Data shown represent the mean values of three experiments ± SD. * p < 0.05 vs. control.