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. 2014 May 21;15(5):9067–9081. doi: 10.3390/ijms15059067

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 4. — (a) Cytotoxicity of 4-arm PEG-SSPHIS copolymer (and 25 kDa PEI as a control) against COS-7 cells at different polymer concentrations; (b) Transfection efficiency and cytotoxicity of 4-arm PEG-SSPHIS in COS-7 cells at 48 h after 4 h co-incubation with the polyplexes of 4-arm PEG-SSPHIS at the mass ratio of 24/1 (2 μg DNA) in the absence of serum and then 44 h-post transfection. Polyplexes of 25 kDa PEI at an optimal ratio of 1/1 was used as a control; (c,d) Transfection efficiency (c) and cytotoxicity (d) of 4-arm PEG-SSPHIS in COS-7 and NIH 3T3 cells at 48 h after 4 h co-incubation with the polyplexes of 4-arm PEG-SSPHIS at the mass ratio of 24/1 (8 μg DNA) in the presence of serum and then 44 h-post transfection. Polyplexes of 25 kDa PEI at an optimal ratio of 1/1 was used as a control.