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. 2014 Jun 6;6:19. doi: 10.1186/1757-4749-6-19

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Copyright © 2014 Bereswill et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Pro-inflammatory cytokine responses in mesenteric lymph nodes following ileitis induction. (A) IFN-γ, B) TNF-α, and (C) nitric oxide protein concentrations were determined in ex vivo biopsies of mesenteric lymph nodes (MLN) derived from C57BL/6 wildtype (WT; black circles) and TLR-9 deficient (TLR-9-/-; white circles) mice seven days following ileitis induction (ILE). Naïve mice served as negative controls (N). Numbers of analyzed mice (in parentheses), means (black bars) and levels of significance (P-values) as compared to the respective groups (determined by Mann–Whitney-U test) are indicated. Data shown were pooled from three independent experiments.