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. 2014 Jun 6;6:19. doi: 10.1186/1757-4749-6-19

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Copyright © 2014 Bereswill et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Flow cytometry analysis of lymphocyte populations in mesenteric lymph nodes following ileitis induction. Lymphocyte populations were isolated from mesenteric lymph nodes (MLN) derived from C57BL/6 wildtype (WT) and TLR-9 deficient (TLR-9-/-) mice before (naïve) and seven days following ileitis induction (ILE) and analyzed by flow cytometry. Frequencies of CD4+ cells producing IFN-γ (right panel), CD4+ CD3+ cells (left panel), as well as CD69+ CD4+ cells (middle panel), are indicated in representative FACS plots (in %). Data shown are representative for three independent experiments.