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. 2014 May 28;14:377. doi: 10.1186/1471-2407-14-377

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Copyright © 2014 Moon et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Changes in methylation and expression of ADHFE1 by ethanol treatment. The methylation and expression changes of ADHFE1 are determined in HT-29, SW480, and DLD-1 cells by treatments with ethanol using QMSP, real-time PCR and immunoblot analysis. A. The methylation status of ADHFE1 is significantly increased by ethanol treatment in 2 CRC cells. B. ADHFE1 expression is diminished by ethanol in 3 CRC cells. C. After treatment with ethanol in CRC cells, the protein expression of ADHFE1 is decreased in a concentration-dependent manner. Expression of GAPDH is used as a loading control. *p-Values of < 0.05 were considered statistically significant; PMR: Percentage of methylated reference.