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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Invest Dermatol. 2014 Feb 14;134(7):1817–1827. doi: 10.1038/jid.2014.94

Figure 1. Mpzl3 knockout (−/−) mice and rc/− mice developed sebaceous gland hypertrophy as seen in the rc/rc mice.

Figure 1

(a) Targeting vector to generate Mpzl3 knockout mice. Exons 2 to 4 were deleted and replaced with a lacZ reporter gene with IRES. The asterisk in exon 3 denotes the point mutation in the rc mice. (b) RT-PCR analysis confirming loss of Mpzl3 RNA expression in Mpzl3 knockout mouse skin. (c) Indirect immunofluorescent staining confirming loss of MPZL3 protein expression in suprabasal keratinocytes (brackets) in Mpzl3 knockout mouse skin (P2). Dotted lines denote the location of the basement membrane. Positive staining in the stratum corneum is an artifact. Scale bars = 50 μm. (d) PCR genotyping of the various Mpzl3 alleles. (e–j) Hematoxylin and eosin (H&E) staining of dorsal skin sections from sex-matched mice (P24). Sebaceous gland hypertrophy was observed in the skin of Mpzl3 −/− mice (g), rc/rc mice (i) and mice heterozygous for both the rc allele and the Mpzl3 knockout allele (rc/−) (j), but not in Mpzl3 +/− (f), rc/+ (h) or wild type (e) mice, indicating that Mpzl3 and rc are allelic. Scale bars = 100 μm. (k, l) Oil Red O staining of lipids in the sebaceous glands (arrows). Mice were 5 months old. Scale bars = 100 μm. (m) Quantitative analysis of sebaceous gland area in skin sections of Mpzl3 −/− and +/+ littermates (P19). Bars=standard error of mean. (n, o) Hyperproliferation of sebocyte precursors in the Mpzl3 −/− skin by PCNA staining (brown, counterstained with hematoxylin). Block arrows point to the many proliferating cells in the Mpzl3 −/− sebaceous glands (o) compared with +/+ skin (n) (P19). Asterisk: enlarged pilary canal in the Mpzl3 −/− skin (g, i, o). Scale bars = 100 μm.