Figure 3. Deficiency of CCR7 expression on DCs in MLN of mice did not impair DC’s ability in inducing donor T cell expression of gut-tropic homing and chemokine receptors.

(A) The MLN from wild-typed and CCR7^−/− C57BL/6 mice were harvested and mononuclear cells were stained for CD11c and CD103. The mean yield of total CD11c⁺, the percentage and yield of CD103⁺ DCs among CD11c^high cells are shown (left). Each group contains 4 mice. (B) CD11c⁺CD103⁺ DCs (0.1×10⁶) in MLN from WT and CCR7^−/− mice were sorted by FACS after magnetic enrichment of CD11c⁺ cells and co-cultured with CD4⁺ or CD8⁺ T cells from BALB/c mice in U-bottom 96-cell plates for 4 days. Expression of α4β7 and CCR9 by the cultured T cells was analyzed by flow cytometry. Mean ± SE is shown from 4 replicate experiments.