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. Author manuscript; available in PMC: 2015 Jun 12.
Published in final edited form as: Cell Rep. 2014 May 22;7(5):1716–1728. doi: 10.1016/j.celrep.2014.04.031

Figure 3. Identification of the antigenic epitope within ROP5IIC that stimulates BTg45Z hybridoma.

Figure 3

A) Schematic representation of the N-terminal and C-terminal deletion constructs generated to identify the antigenic epitope within clone pTg8E6.1. The clone was truncated and missing the 5’UTR and nucleotides encoding the first 222 amino acids. The specific amino acids tested in each construct are shown in parentheses and the antigenic activity region is indicated by the filled gray circle. Open circles represent other putative H-2Db binding epitopes as predicted by the immune epitope database (www.immuneepitope.org) B) BTg45Z lacZ response after an overnight stimulation to different deletion constructs that were transfected in H-2Db expressing APCs C) Sequences of antigenic peptide and its N-terminal extended versions. BTg45Z lacZ response to BMDCs alone or BMDCs that were pulsed with varying concentrations of indicated peptides. See also Figures S2 and S3.