Table 5.
Genes absent from all 4 output pools
| Zero output | Mtb orthologue | Essentiality in H37Rv | Protein function in M. tuberculosis |
|---|---|---|---|
| MAP0298 (MAPK_3470) | Rv1129c | Essential for growth of H37Rv on cholesterol in vitro | Probable transcriptional regulator protein |
| MAP0704 (MAPK_3064) | Rv3121 | Essential for in vitro growth | Probable cytochrome P450 141 Cyp141 |
| MAP0865 (MAPK_2903) | Rv0284 | Essential for in vitro growth | ESX conserved component EccC3 ESX-3 type VII |
| MAP0908c (MAPK_2860) | Rv0966c | Non-essential for in vitro growth | Hypothetical protein |
| MAP0977 (MAPK_2791) | Rv1013 | Required for growth in C57BL/6 J mouse spleen | Putative polyketide synthase Pks16 |
| MAP1031c (MAPK_2737) | Rv2601 | Non-essential for in vitro growth | Probable spermidine synthase SpeE |
| MAP1082c (MAPK_2686) | Rv1936 | Non-essential for in vitro growth | Possible monooxygenase |
| MAP1195c (MAPK_2573) | Rv1467c | Non-essential for in vitro growth | Probable acyl-CoA dehydrogenase FadE15 |
| MAP1236c (MAPK_2532) | Rv2938 | Required for growth in C57BL/6 J mouse spleen | Probable daunorubicin-dim-transport integral membrane protein ABC transporter DrrC |
| MAP1576 (MAPK_2192) | Rv1866 | Non-essential for in vitro growth | Hypothetical protein |
| MAP1584c (MAPK_2184) | Rv2897c | Essential for in vitro growth | Hypothetical protein |
| MAP1601 (MAPK_2167) | n.a. | ||
| MAP1605c (MAPK_2163) | Rv1882c | Non-essential for in vitro growth | Probable short-chain type dehydrogenase/reductase |
| MAP1822c (MAPK_1946) | Rv3903c | Non-essential for in vitro growth | Hypothetical alanine and proline rich protein |
| MAP1835c (MAPK_1933) | Rv2110c | Essential for in vitro growth and encoded proteasome required for persistence in mice | Proteasome beta subunit PrcB |
| MAP1914 (MAPK_1854) | Rv2176 | Non-essential for in vitro growth | Probable transmembrane serine/threonine-protein kinase L PknL (protein kinase L) |
| MAP2008 (MAPK_1760) | Rv2259 | n.d. | S-nitrosomycothiol reductase MscR |
| MAP2385c (MAPK_1383) | Rv3543c | Essential for growth of H37Rv on cholesterol in vitro | Probable acyl-CoA dehydrogenase FadE29 |
| MAP2439c (MAPK_1329) | Rv1321 | Non-essential for in vitro growth | Hypothetical protein |
| MAP2582c (MAPK_1186) | Rv1866 | Non-essential for in vitro growth | Hypothetical protein |
| MAP2964c (MAPK_0804) | Rv1701 | Essential for in vitro growth | Probable integrase/recombinase |
| MAP3070 (MAPK_0698) | Rv3829c | Non-essential for in vitro growth | Probable dehydrogenase |
| MAP3131 (MAPK_0637) | Rv0450c | Essential for in vitro growth | Probable conserved transmembrane transport protein MmpL4 |
| MAP3327c (MAPK_0441) | Rv3529c | Non-essential for in vitro growth | Hypothetical protein |
| MAP3352c (MAPK_0416) | Rv1358 | n.d. | Probable transcriptional regulatory protein |
| MAP3420c (MAPK_0348) | Rv1705c | Non-essential for in vitro growth | PPE family protein PPE22 |
| MAP3699c (MAPK_0069) | Rv0249c | Required for growth in C57BL/6 J mouse spleen | Probable succinate dehydrogenase [membrane anchor subunit] |
| MAP3951c (MAPK_3953) | Rv0457c | Non-essential for in vitro growth | Probable peptidase |
| MAP4117c (MAPK_4119) | Rv0645c | Non-essential for in vitro growth | Methoxy mycolic acid synthase 1 |
M. tuberculosis orthologues are assigned based on the KEGG database (Kyoto Encyclopedia Genes and Genomes; http://www.kegg.jp/[56]) and essentiality information is found on Tuberculist. Gene annotation by Li et al shown in first column with Wynne et al annotation in brackets. n.a. = not applicable, n.d. = no data, PPE = Pro-Pro-Glu.