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. 2013 Dec 21;5(9):2404–2417. doi: 10.18632/oncotarget.1650

Figure 1. FIR/SAP155 and Ku86/DNA-PKcs potentially form a complex in vitro.

Figure 1

(A) DNA-PKcs and SAP155 were examined by pull-down assays in HeLa cells stably expressing FIR–FLAG or FIRΔexon2–FLAG. Typical immunohistochemical stainings with FIR and DNA-PKcs antibodies are shown. (B) Pull-down assays were performed for Ku86 and SAP155 in HeLa and 293T cells transiently expressing FIR–FLAG, FIRΔexon2–FLAG, or PUF60–FLAG. Mock is the negative control with the empty vector. (C) HeLa cells were treated for 24, 48, and 72 h with 5-nM or 20-nM siRNA against SAP155. SAP155, DNA-PKcs, and c-Myc were examined by Western blotting. The negative control was siRNA against GL2.