Figure 5. BLM treatment decreased SAP155 and significantly increased FIR and FIRΔexon2 mRNA expression as well as the FIRΔexon2:FIR ratio in hepatoblastoma (HLE and HLF) cells.

(A) HLE and HLF cells were treated for 48 h with 10 and 100 μg/ml of BLM. whole-cell extracts were analyzed by Western blotting. Mock is untreated cells as negative control. (B) HLE and HLF cells were treated for 48 h with 1, 10, 100 μg/ml of BLM. Total RNAs extracted from the cells were converted to cDNAs and quantitative RT-PCR was performed for FIR and FIRΔexon2 mRNA expression. Mock is untreated cells as negative control.