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. 2013 Dec 21;5(9):2404–2417. doi: 10.18632/oncotarget.1650

Figure 6. Ad-FIRΔexon2 increased Ku86/Ku70, P27Kip1, and c-Myc but not SAP155 in human HCC or HeLa cells.

Figure 6

Ad-FIRΔexon2 was transfected into HepG2, HLE, HeLa, and HLF cell lines. GFP adenovirus (Ad-GFP) was transfected as negative control. Whole-cell lysates were extracted 48 h after transfection and analyzed by Western blotting. (A) Ku70, P27Kip1, and related protein expression was examined in HepG2 and HeLa cell lines after transfection with 1.88 × 109 VP/ml (50 MOI) of Ad-GFP or Ad-FIRΔexon2. (B) c-Myc and SAP155 expression was examined using HLE (lanes 1–3) and HLF (lanes 4–6) cell lines. Lanes 1 and 4 were transfected with 1.88 × 109 VP/ml (50 MOI) of Ad-GFP, lanes 2 and 5 were transfected with 1.88 × 108 VP/ml (5 MOI) of Ad-FIRΔexon2, and lanes 3 and 6 were transfected with 1.88 × 109 VP/ml (50 MOI) of AD-FIRΔexon2. (C) Ku86 and SAP155 expression was examined in HepG2 and HeLa cell lines transfected with 1.88 × 109 VP/ml (50 MOI) of the adenovirus vectors. (D) 100 μg/mL of BLM was treated with/without 20MOI (7.52 × 108 VP/ml) of Ad-GFP, Ad-FIR or Ad-FIRΔexon2 transfection in HLF cells (hepatoblastoma cell line). Ku86/XRCC5, FIR (polyclonal antibody), cyclinE, P27/Kip1, γH2AX and β-actin (internal control) were examined.