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. 2014 Mar 15;5(9):2529–2541. doi: 10.18632/oncotarget.1810

Figure 2. Enhanced antiproliferative effects of Nx in combination with autophagy inhibitors.

Figure 2

A and B. Logarithmically growing Capan-2 and BxPC-3 cells treated with increasing doses of CQ (25μM; panel A) or 3-MA (1mM; panel B). Following 24h incubation, cell proliferation was measured using Cell Titer 96 Aqueous One solution assay. Percent cell proliferation in cells treated with compounds was calculated with respect to solvent control and data was presented as average+sd. Statistical significance between groups was determined using students t-test and p values less than 0.05 was considered significant (* p<0.05, ** p<0.01, and *** p<0.005). Capan-2 (panel C) and BxPC-3 (panel D) cells were treated concurrently with Nx and CQ (25μM) or 3-MA (1mM) for 24h. Following this incubation, cell proliferation was measured as described for panels A and B. Capan-2 (panel E) and BxPC-3 (panel F) cells were treated concurrently with increasing doses of Nx and CQ for 24h and cell proliferation was measured as described above. Combination indices were calculated using isobologram analysis.