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. 2014 Mar 15;5(9):2529–2541. doi: 10.18632/oncotarget.1810

Figure 3. Nx reduces intracellular ROS generation.

Figure 3

A. Logarithmically growing Capan-2 and BxPC-3 cells were treated with 150 μg/ml and 60 μg/ml Nx respectively for 24h. Generation of intracellular ROS was determined by CellROX® deep red reagent (Invitrogen, NY) following manufacturer's instructions. Fold change in intracellular ROS production was calculated relative to untreated samples.B and C. Logarithmically growing Capan-2 (B) and BxPC-3 (C) cells were treated with 150 μg/ml and 60 μg/ml Nx respectively in the presence of N-acetyl cysteine, NAC (5 mM) for 24h. Cell proliferation was measured using the Cell Titer 96 Aqueous One solution assay. Percent cell proliferation in cells treated with compounds was calculated with respect to solvent control and data was presented as average+sd. Statistical significance between groups was determined using students t-test and p values less than 0.05 was considered significant (* p<0.05, and *** p<0.005).