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. 2014 Mar 21;5(9):2562–2574. doi: 10.18632/oncotarget.1812

Figure 7. IGF-1R-mediated let-7b inhibited expression of cell cycle proteins through regulation of the Akt mediated by p-ERK1/2.

Figure 7

A, Western blot analyses in Tca-8113 cells after infection of LV-let-7b and LV-CN and transfection of si-IGF-1R, si-IRS-2 and negative control(si-Ctrl). The amount of phosphorylated Akt (Ser-473), ERK1/2 (T201/Y204) and MEK1/2(Ser217/221) were normalized versus that of total Akt, ERK1/2 and MEK1/2. β-actin served as an internal control. B-C, Statistical results showed the normalized enzyme activities of CDK4/Cyclin A2 in cells treated with LV-let-7b, si-IGF-1R, si-IRS-2 and controls. Each data represented mean ± SEM. *P<0.05.