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. 2014 Mar 22;5(9):2596–2607. doi: 10.18632/oncotarget.1841

Figure 2. Deletion analysis of the CDH11 promoter.

Figure 2

(A) The 3,000-nucleotide CDH11 promoter luciferase reporter plasmid was transfected into MDA-MB-231 or Hs578T cells that were either transfected with HOXC8 siRNA or HOXC8 expression vectors. After 24 hrs, cells were lysed and lysates were analyzed for luciferase activities. pTK-Renilla luciferase plasmid was included in transfection for standardization. Columns, means; bars, SEM; n = 3. *, P < 0.05. (B) A series of 5'-deletion of CDH11 promoter in the pGL3-Basic vector were generated using PCR. TSS: transcription start site. (C) The different lengths of CDH11 promoter reporter plasmids were transfected into MDA-MB-231 or Hs578T cells for 24 hrs and then analyzed for luciferase activities. pTK-Renilla luciferase plasmid was included in transfection for standardization. Columns, means; bars, SEM; n = 3. *, P < 0.05. (D) The 1,000-nucleotide CDH11 promoter reporter plasmid was transfected into MDA-MB-231 or Hs578T cells that were previously transfected with HOXC8 expression vector or HOXC8 siRNA. After 24 hrs, cells were lysed and lysates were analyzed for luciferase activity. pTK-Renilla luciferase plasmid was included in transfection for standardization. Columns, means; bars, SEM; n = 3. *, P < 0.05.