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. 2014 Mar 22;5(9):2596–2607. doi: 10.18632/oncotarget.1841

Figure 3. Region containing nucleotides −196~−191 is essential for HOXC8-induced CDH11 promoter activation.

Figure 3

(A) The positions of three putative HOX protein binding sites in CDH11 promoter region. TSS, transcription start site. (B) MDA-MB-231 or Hs578T cells were transfected with wild-type or various mutant CDH11 promoter reporter plasmid for 24 hrs followed by the analysis of luciferase activity. pTK-Renilla luciferase plasmid was included in transfection for standardization. Columns, means; bars, SEM; n = 3. *, P < 0.05. (C) The reporter plasmid containing 1,000-nucleotide wild-type CDH11 promoter or CDH11 promoter containing mutation at nucleotides −196~−191 was transfected into MDA-MB-231 or Hs578T cells that were previously transfected with HOXC8 expression vector (left panel) or HOXC8 siRNA (right panel). After 24 hrs, cells were lysed and lysates were analyzed for luciferase activities. pTK-Renilla luciferase plasmid was included in transfection for standardization. Columns, means; bars, SEM; n = 3. *, P < 0.05.