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| Copy number of GLS2 | Non-Tumor | Tumor |
|---|---|---|
| 1 | 0 | 1 |
| 2 | 21 | 20 |
| Total | 21 | 21 |
LOH of GLS2 in HCCs was detected by Taqman copy number assays. Three different copy number assays, which measured the 5’, 3’ and the central region of the GLS2 gene, respectively, were employed and the same results were obtained. Primary HCCs and their matched adjacent non-tumor liver samples (n=21; MD Anderson Cancer Center) were used for assays.
