Figure 4. ISO treatment deactivated MKK7/JNK cascade.

(A and B) Cl41 cells were pretreated with ISO (50 μM) for 30 min and then co-incubated with ISO and TPA/ EGF (40 ng /ml) for indicated time periods. The total cell extracts were subjected to Western blot analysis with specific antibodies against p-ERK, ERK, p-p38, p38, p-JNK, JNK, p-MKK4, MKK4, p-MKK7, and MKK7. β-Actin protein was used as protein loading control. Data are representative of three independent experiments.