Figure 3. Increased proliferation of sympathetic neurons at P0 in Alk^F1178L KI mice.

(A) GSEA of cell cycle and G1/S transition pathway genes in transcriptomic data of Wt and mutated ganglia at P0. The normalized enrichment score (NES) and the nominal p-value are indicated. (B) Quantification of double positive cells for islet1 and ki67 revealed an increased proliferation at P0 in ganglia of heterozygotes (Hz) and homozygotes (Ho) KI Alk^F1178L mice. Bonferroni Multiple Comparisons Tests were used to evaluate differences between the groups (n=4 samples in each group). Error bars represent standard deviation. (C) Expression of the Ret gene in sympathetic ganglia at birth. (D) Heatmap of genes belonging to the “Mitosis” category (GO:0007067) in P0 and P18 mutated and Wt ganglia.