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. 2014 Apr 2;5(9):2688–2702. doi: 10.18632/oncotarget.1883

Figure 5. Ret is upregulated in murine and human NB exhibiting Alk activation.

Figure 5

(A) Unsupervised hierarchical clustering using the top 5% genes with the highest IQR clearly separates MYCN and MYCN/Alk tumors. (B) The list of the top 50 upregulated genes in MYCN/Alk tumors is shown together with a heatmap. It includes the Ret, Vgf and Vip genes. (C) Expression level of the RET gene in primary tumors with (n=16) and without ALK activation (n=144) as determined by Schulte and colleagues [29]. (D) RET down-regulation in CLB-Ga upon TAE-684 treatment (blue). Untreated cells are shown in red. (E) Decreased expression of RET upon TAE-684 treatment in human NB cell lines with amplified ALK (blue), F1174L ALK (green) or R1275Q ALK (red). Cell lines with Wt ALK appear in grey. C: Control cells; T: TAE-684 treated cells. 1: NB-1; 2: Kelly; 3: SK-N-SH; 4: SMS-KNCR; 5: CLB-Ga; 6: LAN5; 7: UKF-NB-3; 8: IMR-32; 9: NGP; 10: SK-N-AS. P values were calculated using one-sided Wilcox-test.