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. 2014 May 1;5(9):2714–2772. doi: 10.18632/oncotarget.1931

Figure 1. Protease dependent generation of LynΔN.

Figure 1

(a) K562 cells were transfected with a Lyn-GFP expressing vector. 24 hours after transfection, the cells were treated with 1μM imatinib for the indicated times. The cells were then lysed and resolved by SDS-PAGE for Western blot (WB) analysis. Western blot analysis reveals a faster migrating species after 48 hours of imatinib treatment. For WB analysis the polyclonal rabbit primary antibody used was either anti-GFP or anti-PARP (b) Schematic depiction of the generation of LynΔN by either caspase cleavage after Asp18 or as a recombinant ubiquitin fusion. Both methods of generating LynΔN in cells results in an N-terminal leucine.