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. 2014 Apr 7;5(9):2750–2760. doi: 10.18632/oncotarget.1887

Figure 1. The mAb 4E1 affects cell proliferation, migration, and in vitro sprouting of human endothelial cells.

Figure 1

A: Cell proliferation expressed as thymidine uptake in HUVEC. Cells were grown in 96-well-plates, serum starved, and induced to proliferate with complete medium (induct.) in the presence of different concentrations of 4E1 or unrelated purified antibodies (NC, 500 nM). B: Migration assay on HUVEC in the presence of 4E1 (500 nM) or unrelated purified antibodies (NC, 500 nM). Cells were grown in growth factor-depleted culture medium and plated in Boyden chambers. Chemotaxis was stimulated with 10 ng/ml VEGF (VEGF) or complete medium (M199). Migratory cells were stained and counted under a light microscope. C: Sprouting of HUVEC spheroids embedded into collagen gels in the absence (NT) or presence of 10 ng/ml VEGF (VEGF). NC, unrelated purified antibodies (500 nM), mAb 4E1 (500 nM). A representative experiment is shown (original magnification, x40). Data represent the ± SD of three-five independent experiments each in triplicate.