Figure 5. The mAb 4E1 impairs endothelial cell adhesion on different substrates.

Gelatin (gel), laminin (lam) and collagen (coll) adhesive substrates were used for coating. HUVEC, grown in complete medium, were biochemically detached, incubated in the presence of different concentrations of 4E1 (50, 250, and 500 nM) or unrelated antibodies (500 nM, NC) for 15 min in ice and allowed to adhere on substrate-coated surfaces for 10 min. Fixed cells were stained with crystal violet solution, and the optical density values were expressed as relative cell adhesion. Data represent the ± SD of four independent experiments each in triplicate.