Figure 2. Comparisons of USP4, S1P1 and E-cadherin levels in liver-tumor cell lines.

(A) Immunoblottings for USP4, S1P1 and E-cadherin. E-cadherin and vimentin were used as the criteria to determine epithelial- or mesenchymal-phenotype. (B) qRT-PCR assays for USP4, S1P1 and E-cadherin transcripts. (C) Correlation analyses. The relative USP4 or S1P1 mRNA levels were plotted against those of E-cadherin (E-cadherin in HepG2=1). Actin was used as control. For B and C, data represented the mean ± S.E. of at least 3 separate experiments.