Figure 7. Analyses of miR-148a and its target expression levels in original HCC or engrafted tumors.
(A) Clinical and pathological characteristics of donor patients. miR-148a levels were assessed using qRT-PCR assays and were normalized relative to U6 small RNA. miR levels in tumors were compared with NT samples. One or two paraffin blocks were made from G1 or G2 tumors, and the rest samples were used for tumor passages.(B) Immunohistochemical analyses for USP4 or S1P1 in original HCC or engrafted tumors. Shown above are the figures obtained from patient #2 and #3 (×200). The results from patient #1 and #2 were comparable to each other.(C) Correlation analyses between miR-148a and USP4 (or S1P1). U6 small RNA was used as control. (NT=1)
