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. 2014 May 1;5(9):2827–2838. doi: 10.18632/oncotarget.1940

Figure 3. MMP-3 responsive region of the Snail promoter contains an NF-κB binding site.

Figure 3

(A) Luciferase reporters under control of mouse Snail promoter region −525, −300 or −100 bp upstream of Snail translation start site, respectively, reveal MMP-3-responsive element localized in the −300/−100 bp region. (B) Further promoter reporter deletions identify the MMP-3 responsive region to be localized −200/−100 bp upstream of the Snail translation start site. Schematic representation of the mouse Snail promoter region (top) and deletion mutants tested (bottom), showing the position of potential regulatory elements. (C) Diagram showing mutation of the candidate NF-κB binding site in the Snail promoter reporter. (D) Mutation of the candidate NF-κB binding site in the S300 reporter inhibits its induction by MMP-3. (A), (B), (D) Error bars, SE of triplicates.