Figure 2. Apoptosis and cell cycle arrest induced by SeC or/and DOX.

A. DOX triggers G2/M cell cycle arrest in HepG2 cells in dose-dependent manner. HepG2 cell were pre-treated with 10 μM SeC for 24 h and co-treated with 100 nM DOX for 24 h.

B. Combined treatment with SeC and DOX triggers apoptosis and cycle arrest. Cells after treatment were collected and stained with PI solution after fixation by 70% ethanol. Then cells was analyzed by flow cytometric analysis. Apoptotic cells with hypodiploid DNA content were measured by quantifying the sub-G1 peak. Statistical analysis of apoptotic cell (C) and cell cycle population (E and F). D. Caspase-3 activity. Cell after treatment were lysed and the cell proteins was used to determine caspase-3 activity using specific fluorescence substrates.