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. 2014 May 2;3(6):418–430. doi: 10.1242/bio.20147021

Fig. 3. RanBPM localizes to aggresomes with HDAC6 in response to DNA damage.

Fig. 3.

Hela cells stably expressing control shRNA or RanBPM shRNA were fixed 72 h following 1 h treatment with etoposide (2 µM) or vehicle (DMSO). Cells were processed for immunostaining with antibodies to RanBPM and HDAC6 and mounted with DAPI. (A) Representative images of etoposide-treated Hela control cell showing colocalization of RanBPM and HDAC6 in perinuclear aggresome. (B) Quantification of aggresome formation in response to etoposide. At least 100 cells per experiment were scored for HDAC6-containing aggresomes. Results are averaged from three different experiments, with error bars indicating SE. Asterisks indicate statistical significance between treatments and cell lines, P<0.005 (**); P<0.05 (*). Scale bar: 10 µm.