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(A) Measurement of GRASP65, GRASP55 and GM130 mRNAs by real time RT PCR. (B,B′) Immunofluorescence visualization of GRASP65, GRASP55 and GM130 in wild type (B) and GRASP65[LacZ] homozygous (B′) immortalized MEFs. (C,C′) Golgi profiles on ultrathin epon sections (flat embedding) of wild type (B) and GRASP65[LacZ] homozygous (B′) immortalized MEFs. Scale bars: 2 µm (B,B′), 500 nm (C,C′).
