Skip to main content
. Author manuscript; available in PMC: 2014 Oct 10.
Published in final edited form as: Mol Cell. 2014 Mar 6;54(1):56–66. doi: 10.1016/j.molcel.2014.02.004

Figure 2. RHS6 is a critical region that mediates interchromosomal associations of the Th2 and IL-17 loci in naïve T cells.

Figure 2

(A) Frequency of 3C PCR chimeric products obtained with one primer annealing to a region of the Th2 locus (RHS2, RHS6 or RHS7) and the other primer annealing to a region of the IL-17 locus (CNS87, CNS64, the IL-17A promoter, CNS164, the IL-17F exon or the IL-17F promoter). For normalization, BAC DNA containing the Th2 locus or the IL-17 locus was digested, ligated and used as a 3C PCR template. (B) Frequency 3C PCR chimeric products between RHS6 and the IL-17A promoter or CNS164 in naïve T cells, Th2 cells, Th17 cells and MEFs. (C) Frequency 3C PCR chimeric products between RHS7 and the IL-17A promoter or CNS164 in naïve T cells, Th2 cells, Th17 cells and MEFs. (D) Cumulative frequency plots of normalized distances between the Th2 locus and the IL-17 locus (left) or the IFN-γ locus and Th2 locus (right) in WT and RHS6 KO naïve T cells (N=200). (E) Percentage of WT and RHS6 KO naïve T cells showing an interchromosomal separation equal to or below 1.5μm. Data are representative of at least three independent experiments and represented as mean +/− SEM. The KS-test was performed and P-value < 0.01 for (D) was obtained. See also Figure S2.