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. Author manuscript; available in PMC: 2014 Oct 10.
Published in final edited form as: Mol Cell. 2014 Mar 6;54(1):56–66. doi: 10.1016/j.molcel.2014.02.004

Figure 3. Oct-1 is bound at RHS6 and at the IL-17 promoter in naïve T cells.

Figure 3

(A) ChIP-qPCR for Oct-1 on RHS6, the IL-17A promoter and the Taf12 promoter in naïve, Th2 and Th17 cells. The results were averaged for three independent experiments. The Student t-test was performed (**P < 0.01, ***P < 0.001). (B) Cumulative frequency plots of normalized distances between the Th2 and IL-17 loci (left) or between the IFN-γ and Th2 loci (right) in RHS6ΔOct-1 WT and RHS6ΔOct-1 KO naïve T cells (N=200). (C) Percentage of RHS6ΔOct-1 WT and RHS6ΔOct-1 KO naïve T cells showing an interchromosomal separation equal to or below 1.5μm. (D) Cumulative frequency plots of normalized distances between the Th2 and IL-17 loci (left) or between the IFN-γ and Th2 loci (right) in C57BL/6, IL-17A-GFP and IL-17A-GFP Oct-1 binding site mutant naïve T cells (N=100) (E) Percentage of C57BL/6, IL-17A-GFP and IL-17A-GFP Oct-1 binding site mutant naïve T cells showing an interchromosomal separation equal to or below 1.5μm. Data are representative of at least two independent experiments and represented as mean +/− SEM. The KS-test was performed and P-value < 0.05 for (B) and (D) was obtained. See also Figure S3.