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Ischemic OPN−/−-hearts show different expression contractile elements. mRNA-expression of (a) α-myosin heavy chain (α-MHC), (b) β-MHC, (c) skeletal actin, (d) cardiac actin, and (e) desmin in OPN−/−-mice compared to WT-mice during repetitive I/R. n = 8-9/group. RT-qPCR using Taqman and mRNA-expression is related to controls and GAPDH using comparative ΔΔCt-method. Bracket indicates P ≤ 0.05 between genotypes; ∗ indicates P ≤ 0.05 versus respective shams.
