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. 2014 May 29;2014:124063. doi: 10.1155/2014/124063

Figure 7.

Figure 7

Expression of remodeling markers in myofibroblasts in vitro. Cultured myofibroblasts from WT- and OPN−/−-hearts were stimulated with human TGF-β1 or PBS as control under normoxia (21% O2) or hypoxia (2% O2). mRNA-expression of (a) OPN-1 in WT-cells only. mRNA-expression of (b) galectin-3, (c) CCL2, (d) tenascin-C (TNC), (e) MMP-9, (f) MMP-12, and (g) TIMP-1. n = 5–7/group. RT-qPCR using Taqman and mRNA-expression is related to controls and 18 S (in vitro) using comparative ΔΔCt-method. Bracket indicates P ≤ 0.05 between genotypes; ∗ indicates P ≤ 0.05 versus 6 h + PBS normoxia group (control).