Table 1. Lamin A/C phosphorylation sites identified by LC-MS/MS from interphase Hela cells.

See supplementary material Fig. S2 for two representative examples of phosphorylation site characterization. The site identification also involved data from experiments that combined phosphopeptide mapping, mass spectrometry and manual Edman degradation (supplementary material Fig. S1). Additionally, residue Ser423 was identified as a tryptic peptide 421-LEpSTESR-427 in a separate experiment that involved ³²P-labeled peptides that were separated by high-performance liquid chromatography and identified by mass spectrometry (data not shown). All results are summarized in Fig. 1A. A lowercase p indicates that the following residue was identified as a phosphorylation site (in bold).